Get familiar with our
newest innovation:
NL5 & NL5+
Fast & deep 3D live cell imaging
High resolution & low phototoxicity
About the NL5 series
Fast & deep 3D live cell imaging
Our line scanning confocal microscope NL5 series takes the best of two worlds; point scanners and spinning disc, leading to a system with options to do confocal, widefield or brightfield observations.
Phototoxicity is a central problem in the application of confocal microscopy for live cell imaging. To overcome these challenges, we developed the second generation of fast line-scanning confocal technologies. Using a line scanner instead of a point scanner, a confocal microscope provides high temporal resolution, at the same time reducing the phototoxicity and allowing long-term imaging. A slit pinhole, together with an extremely sensitive sCMOS camera as a detector, makes the NL5 series the perfect tool for fast and deep 3D live cell imaging.
Max projection of a stack in time with 1000 frames per color HUVEC labeled for SPY505-DNA, SPY555-Actin and SPY650-Tubulin Sample courtesy of Philippa Phelp (Boon’s Lab) Physiology department. VUmc Amsterdam
Volvox autofluorescence 561 excitation channel. Image acquired 90 microns away from the coverslip 100x 1.5 objective was used with both systems
Contrary to spinning-disk systems, another advantage of the NL5 series and its slit pinhole is that it does not lead to pinhole crosstalk when imaging deep within the sample. The NL5 series reaches 240 nm lateral resolution which can be easily converted into 170 nm with on the fly deconvolution.
NL5 is an excellent add-on to any research microscope. It allows for converting a wide-field system into a fast scanning confocal with high resolution and sensitivity.
Perks of NL5 & NL5+
- Fast line scanning confocal microscope
- High resolution and low phototoxicity
- Ideal for deep 3D live cell imaging
- Compact and very easy to use
- Convert your existing widefield fluorescence microscope into a fast and highly sensitive confocal 3D imaging microscope with NL5
Discover the way NL5 improves your imaging experience
Discover our solutions
Clever design for brighter images and even lower laser power
NL5
NL5 is a fast line-scanning Confocal system with high sensitivity and resolution. It allows to quickly acquire multiple multicolor images, with minimal phototoxicity.
NL5+
NL5+ has an additional filter, placed on top of your confocal system. Get high-contrast images from thicker specimens and create long time-lapse experiments for the imaging of low-signal samples.
Discover the NL5 system
that meets your needs
To explain the differences easily
NL5 | NL5+ | |
|---|---|---|
Detector | Camera (sCMOS) | Camera (sCMOS) |
Resolution in real time | 170 nm * | 170 nm * |
Detector sensitivity | up to 96% QE | up to 96% QE |
FOV | FOV 18mm (FN18); 330×330 µm (40x objective) | FOV 18mm (FN18); 330×330 µm (40x objective) |
Speed in line scanning | up to 75 fps for 2048×512 px and up to
55 fps for 2048×2048 px** | up to 75 fps for 2048×512 px and up to
55 fps for 2048×2048 px** |
Speed in fixed line mode | 500 fps and more (camera limited) | 500 fps and more (camera limited) |
Wavelength | VIS (400-700 nm) | VIS (400-700 nm) |
Software | Micromanager, Inscoper, Volocity, NIS
Elements | Micromanager, Inscoper, Volocity, NIS
Elements |
Deconvolution with | Microvolution (real time), SVI Huygens
(postprocessing) | Microvolution (real time), SVI Huygens
(postprocessing) |
Modalities | Widefield and Confocal (motorized) | Widefield and Confocal (motorized) |
Emission filter | 1 position (standard with Quadband) | 6 positions |
Weight | 4kg | 4.5kg |
See for yourself how we improve your experience
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