Our Re-scan Confocal
Microscope systems
Camera-Based Confocal Technology
Re-scan Confocal Microscope: sharp and sensitive
Re-scan Confocal Microscopy (RCM) is a super-resolution technique based on a standard confocal extended with an optical re-scanning unit that projects the image directly on a CCD or sCMOS camera (Luca et al., 2013).
By using a sensitive camera as a detector, the quantum efficiency (QE) of RCM increases up to 95% in the visible range of light (VIS) and 67% in near-infrared (NIR) light. Traditional point scanning confocal systems use Photomultiplier Tubes (PMTs) as a detector. The sensitivity of a PMT is maximum 45%. RCM strongly increases sensitivity while maintaining the confocal sectioning capability. The re-scan technology allows the development of images with a 40% increase in resolution, without closing the pinhole. Therefore, more photons are captured, and the signal-to-noise ratio is four times higher in RCM than in standard PMT-based confocal microscopes. This results in 170 nm resolution images with an extremely high signal-to-noise ratio. Combining RCM with deconvolution, the resolution is further improved to 120 nm.
Discover the way RCM improves your imaging experience
Watch this explanatory video to discover the principle.
HO1N1 cells with mitochondria RFP
Perks of Re-scan Confocal Microscopes
- It is excellent for biological applications where a combination of super-resolution and high sensitivity is required.
- No need for reconstruction software to increase the resolution. RCM achieves 170nm in the RAW image, which can be further improved to 120nm, using easy-to-use deconvolution software.
- The clever design of RCM makes these products robust, versatile, and extremely easy to use.
- Convert your existing widefield fluorescence microscope into a super-resolution and high sensitivity confocal 3D imaging system with RCM.
Discover the way RCM improves your imaging experience
Discover our solutions
Ingenious design for the best live cell imaging you can get
RCM1
RCM1 is a highly sensitive super-resolution confocal system. It’s capable of reaching a 120nm resolution after deconvolution (170nm raw). Study living cells in high detail with minimal phototoxicity and photobleaching.
RCM2
RCM2 is, like the RCM1, a highly sensitive super-resolution confocal system. It uses digital scanning technology and bigger optics, which allows for brighter images and even lower laser power. Capture more biology with the same resolution.
RCM2.5
RCM2.5 is our upgraded version of the RCM2, with a 5th additional laser. This version utilizes up to 5 lasers, extending over the Visible and Near-Infrared (NIR). Moreover, RCM2.5 enables you to use the latest advances in NIR dye development and look much deeper into your specimen. Experience complete experimental freedom and flexibility with our RCM2.5.Â
NL5
NL5 is a fast line-scanning Confocal system with high sensitivity and resolution. It allows to quickly acquire multiple multicolor images, with minimal phototoxicity.
Discover the RCM system
that meets your needs
To explain the differences easily
Let our images do the talking
RCM1 | RCM2 | RCM2.5 | |
|---|---|---|---|
Detector | Camera (sCMOS) | Camera (sCMOS) | Camera (sCMOS) |
Resolution in real time | 120 nm * | 120 nm * | 120 nm * |
Detector Sensitivity | up to 96% QE | up to 96% QE | up to 96% QE |
FOV | FN12: 150×150 μm (60x objective), FN18 when not using super resolution | FN12: 220×220 μm (40x objective), FN18 when not using super resolution | FN12: 220×220 μm (40x objective), FN18 when not using super resolution |
Optimized for super resolution | 100x, 60x (high NA) | 100x, 60x, 40x (high NA) | 100x, 60x, 40x (high NA) |
Speed | 1fps @ 512 x 512 px | 2 fps at 512 x 512 px, 15 fps in sprint level max at 256 x 256 px | 2 fps at 512 x 512 px, 15 fps in sprint level max at 256 x 256 px |
Wavelength | VIS or NIR | VIS | VIS+NIR |
Software | Micromanager, NIS Elements, Volocity | Micromanager, Inscoper, Volocity, NIS Elements, SDK available for integration on request | Micromanager, Inscoper, Volocity, NIS Elements, SDK available for integration on request |
Deconvolution with | Microvolution (real time), SVI Huygens | Microvolution (real time), SVI Huygens | Microvolution (real time), SVI Huygens |
Emission filters | standard quad band, optional external single band filter wheel | standard quad band, optional external single band filter wheel | standard penta band, optional external single band filter wheel |
*after deconvolution, raw image = 170nm
Take a look at our images
All
RCM1
RCM2
See for yourself how we improve your experience
Request your
personalized demo
- Your online demo will be shown in only 45-60 minutes
- Our experts will lead you through our products performance
- We will contact you and together, we pick a day and time
